Stiansen EN, Lund JS, Rortveit T, Rustan AC: Impact of dietary n-3 and n-6 fatty acids on fatty acid desaturation in rat liver. Biochim Biophys Acta 1991, 1082:57?2. 46. Igarashi M, Ma K, Chang L, Bell JM, Rapoport SI: Dietary n-3 PUFA deprivation for 15 weeks upregulates elongase and desaturase expression in rat liver but not brain. J Lipid Res 2007, 48:2463?470. 47. Tu WC, Cook-Johnson RJ, James MJ, Muhlhausler BS, Gibson RA: Omega-3 lengthy chain fatty acid synthesis is regulated extra by substrate levels than gene expression. Prostaglandins Leukot Essent Fatty Acids 2010, 83:61?eight.doi:ten.1186/1476-511X-12-147 Cite this short article as: Casey et al.: Effect of stearidonic acid-enriched soybean oil on fatty acid profile and metabolic parameters in lean and obese Zucker rats. Lipids in Health and Disease 2013 12:147.
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 290, NO. three, pp. 1319 ?331, January 16, 2015 ?2015 by The American Society for Biochemistry and Molecular Biology, Inc. Published inside the U.S.A.Involvement with the Na /Ca2 exchanger isoform 1 (NCX1) in Neuronal Development Issue (NGF)-induced Neuronal Differentiation via Ca2 -dependent Akt PhosphorylationReceived for publication, July 7, 2014, and in revised type, NK3 Inhibitor drug November 21, 2014 Published, JBC Papers in Press, November 21, 2014, DOI ten.1074/jbc.M114.Agnese Secondo1, Alba Esposito1, Rossana Sirabella?, Francesca Boscia, Anna Pannaccione, Pasquale Molinaro, Maria Cantile, Roselia Ciccone, Maria Jos?Sisalli, Antonella Scorziello, Gianfranco Di Renzo, and Lucio Annunziato? From the Department of Neuroscience and Reproductive and Odontostomatological Sciences, School of Medicine, “Federico II” University of Naples, Through Sergio Pansini five, Naples 80131, Italy plus the �Fondazione Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) SDN, Naples 80143, ItalyBackground: NCX1 regulates intracellular Ca2 and Na homeostasis in neurons. Final results: The overexpression of NCX1 induced neuronal differentiation through Akt also as NGF exposure. NCX1 knockdown prevented NGF-induced neurite outgrowth. Conclusion: NCX1 TrkC Inhibitor supplier participates in neuronal differentiation by ionic regulation and Akt phosphorylation. Significance: Studying how NCX1 participates in neurite outgrowth will improve the expertise of neuronal differentiation. NGF induces neuronal differentiation by modulating [Ca2 ]i. On the other hand, the role on the 3 isoforms of the most important Ca2 -extruding program, the Na /Ca2 exchanger (NCX), in NGF-induced differentiation remains unexplored. We investigated irrespective of whether NCX1, NCX2, and NCX3 isoforms could play a relevant function in neuronal differentiation via the modulation of [Ca2 ]i plus the Akt pathway. NGF caused progressive neurite elongation; a substantial raise from the well-known marker of growth cones, GAP-43; and an enhancement of endoplasmic reticulum (ER) Ca2 content material and of Akt phosphorylation through an early activation of ERK1/2. Interestingly, for the duration of NGF-induced differentiation, the NCX1 protein level enhanced, NCX3 decreased, and NCX2 remained unaffected. In the same time, NCX total activity improved. Moreover, NCX1 colocalized and coimmunoprecipitated with GAP-43, and NCX1 silencing prevented NGF-induced effects on GAP-43 expression, Akt phosphorylation, and neurite outgrowth. Alternatively, the overexpression of its neuronal splicing isoform, NCX1.4, even in the absence of NGF, induced an increase in Akt phosphorylation and GAP-43 protein expression. Interestingly, tetrodotoxin-sensitive Na curren.