ACE2 in enterocytes), SLC7A9 (which codes for an L-DOPA influx transporter) and SLC16A10 (which codes for an L-DOPA efflux transporter). From the entire set of information (n = six, two control samples, two samples at 24 h post-infection and two samples at 60 h post infection), we could extract expression values for 11 out of 14 genes of interest. We then employed the Pearson’s correlation test to evaluate the co-expression hyperlinks among these genes and ACE2. We identified that eight important genes involved inside the metabolism of dopamine and/or trace amines exhibited statistically considerable co-expression hyperlinks with ACE2 across all experimental circumstances. Of note, by far the most robust correlation hyperlink was observed for MAOB, followed by SLC7A9 and SULT1A1 (Table three).Int. J. Mol. Sci. 2021, 22,tern. Additionally expected, the L-DOPA efflux transporters SLC3A2 and SLC7A8 have been detected in the basolateral membrane of enterocytes. A low and ALK1 custom synthesis diffuse staining pattern was observed for SLC16A10. Ultimately, no TH staining could be detected (Figure S1), in accordance with genomics analyses. Depending on these mined data, a scheme summarizing the predicted dopamine/trace amines metabolic pathways taking place in human enterocytes 6 of 16 is shown in Figure two.Figure two. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking place in human Figure 2. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking place in huenterocytes of of smaller intestine. This scheme is depending on the mining of human expression atlases and on previously man enterocytesthe the modest intestine. This scheme is primarily based onthe mining of human expression atlases and on previously publishedbiochemical and/or functional information obtained in intestinal or non-intestinal cells. The molecules integrated within this published biochemical and/or functional information obtained in intestinal or non-intestinal cells. The molecules included within this scheme comprise: angiotensin-converting enzyme (ACE2), solute carrier loved ones six member 19 (SLC6A19), solute carrier scheme comprise: angiotensin-converting enzyme two two (ACE2), solute carrier loved ones six member 19 (SLC6A19), solute carrier loved ones 33member 11(SLC3A1), solute carrier family 77member 99(SLC7A9), dopa-decarboxylase (DDC), sulfotransferase family member (SLC3A1), solute carrier AT1 Receptor Compound household member (SLC7A9), dopa-decarboxylase (DDC), sulfotransferase loved ones 1A member 11 (SULT1A1),sulfotransferase household 1A member 22 (SULT1A2),sulfotransferase loved ones 1A member 33 loved ones 1A member (SULT1A1), sulfotransferase household 1A member (SULT1A2), sulfotransferase family 1A member (SULT1A3), cytochrome P450 loved ones two subfamily D member 6 (CYP2D6), monoamine oxidase A (MAOA), monoamine oxidase B (MAOB), solute carrier family members three member 2 (SLC3A2), solute carrier family members 7 member eight (SLC7A8) and solute carrier family members six member 10 (SLC16A10). Table three. Correlation evaluation of ACE2 mRNA levels with crucial genes with the dopamine/trace amines metabolic pathways in SARS-CoV2-infected human enterocytes. DDC 0.84 0.035 MAOA 0.86 0.025 MAOB 0.96 0.001 SULT1A1 0.92 0.007 SLC7A9 0.95 0.003 SLC3A1 0.87 0.02 SLC6A19 0.88 0.017 SLC3A2 0.9 0.Expression information have been extracted from Lamers et al. [34] plus the Pearson’s test was applied to assess correlation coefficient (r, upper line) and statistical significance (p-value, reduce line)) involving ACE2 and genes of interest. Gene symbols: dopa-decarboxylase (DDC), monoamine oxidase A (MAOA), monoamine oxidase B (MAOB), solute carr