oic acid Benzoic acid Caffeic acid Catechol Chlorogenic acid Cinnamic acid CD40 Purity & Documentation Coumarin Ellagic acid e-Vanillic acid Ferulic acid Gallic acid Iso-ferulic acid -Coumaric acid p-Coumaric acid p-Hydroxybenzoic acid Protocatechuic acid Pyrogallol Rosmarinic acid Salicylic acid Sinapic acid Syringic acid Vanillic acid Apigenin-7-glucoside D-Catechin Epicatechin Kaempferol Myricetin Quercetin Rutin Ethanolic Extract (KEE) (mg 100 g-1 ) six.621 0.094 1.854 3.440 1.811 two.884 28.704 1.083 3.326 0.192 2.410 0.434 1.627 0.184 0.539 Aqueous Extract (KAE) (mg one hundred g-1 ) 0.042 0.012 0.005 0.725 2.526 0.136 0.001 0.036 0.039 0.443 0.037 0.041 0.005 0.039 0.009 0.223 0.454 1.589 0.089 1.959 1.406 0.256 0.193 -1 2 3 four five 6 7 eight 9 ten 11 12 13 14 15 16 17 18 19 20 21 22 23 1 2 three 4 five 6Phenolic acidsFlavonoidsNotes: KEE: Anastatica hierochuntica ethanolic extract; KAE: Anastatica hierochuntica aaqueous extract.three.three. Serum Creatinine, Urea, K, Total Protein, and Albumin Levels CCl4 injection substantially raised serum creatinine, urea, and k levels in GII rats when compared to 12-LOX web control rats (GI). Conversely, total protein and albumin levels had been considerably decreased in CCl4 -treated rats (Table three). Vit. E + Se and a. hierochuntica extracts (G III, IV, V, and VI) substantially lowered the alterations in creatinine and urea triggered by CCl4 injection, whilst they elevated albumin and total proteins to be close to standard values in GI (Table three). Serum k level was markedly improved in CCl4 -treated rats (GII) when in comparison with GI (Table three). The injection of vit. E + Se and administration of A. hierochuntica alcoholic and aqueous extracts (G IV, V, and VI) was also positively boost the k level when in comparison to GI (Table three).Nutrients 2021, 13,7 ofTable 3. Impact of oral administration of A. hierochuntica extracts on biochemical kidney markers in CCl4 -induced toxicity in rats (mean SE), n = six. Kidney Functions GI Creatinine (mg Urea (mg dL-1 ) K (mEq L-1 ) Total proteins (g dL-1 ) Albumin (g dL-1 ) dL-1 ) 0.88 0.09 77.59 two.60 a four.18 0.21 a eight.71 0.92 c 3.91 0.13 baExperimental Groups GII 1.30 0.11 117.00 three.98 b 5.55 0.68 bc five.04 0.36 a three.28 0.09 abGIII 0.87 0.11 77.53 ten.11 a 4.57 0.23 ab 7.54 0.45 b three.79 0.31 baGIV 0.99 0.07 73.60 five.35 a four.78 0.21 b 7.89 0.44 bc 3.68 0.16 baGV 1.08 0.03 78.65 12.69 a five.00 0.21 b eight.59 0.18 c four.34 0.17 caGVI 0.91 0.11 a 70.33 8.37 a five.48 0.23 c 5.89 1.43 ab 3.71 0.14 bGI: handle unfavorable group, GII: handle good group received CCl4 (i.p.), GIII: CCl4 -rats received 50 mg kg-1 vit. E + Se twice per week (i.m.), GIV: CCl4 -rats received KEE as 250 mg kg-1 per oral (p.o.) daily, GV: CCl4 -rats received KAE as 250 mg kg-1 (p.o.) each day and GVI: CCl4 -rats received KEE + KAE (1:1) as 250 mg kg-1 (p.o.) day-to-day. a : values with all the similar superscript letter inside the exact same raw are certainly not considerably distinct at p 0.05.3.four. Renal Antioxidant Biomarkers As shown in Table four, administration of CCl4 drastically reduced SOD and GSH levels and elevated the MDA level in GII kidney homogenate tissue. Even so, when in comparison with GI, rats treated with each vit. E + Se and a. hierochuntica extracts (GIII, VI, V, and VI) exhibited a substantial improvement within the activity of antioxidant enzymes SOD and GSH, at the same time as a reduction in MDA levels (Table 4). A. hierochuntica alcoholic extract (GIV) outperformed A. hierochuntica aqueous extract (GV) and combined A. hierochuntica alcoholic and aqueous extracts in attenuating antioxidant levels, and combating the autoxi