A “redox state marker” on the ferric low spin heme in may be deemed to be amode, vibrations of methineferric low spin heme in cyt c, assignedthe the v19 “redox state marker” with the bridges (CC, CCH bonds) and to CC bond v19 mode, vibrations of α4β7 custom synthesis methineshow that human breast bonds) plus the CC bond. Our benefits bridges (CC CC cancer and brain tumor demonstrate a Our final results show that human breast cancer and brain tumor demonstrate a redox imbalance compared to regular tissues. We discovered that concentration of cytochrom imbalance compared to typical tissues. We identified that concentration ofof the 1584 cmis Raman upregulated in brain and breast cancers. The intensities -cytochrome c -1 1 upregulated in brain and breastthe amount of intensities of cytochrome c increases signal corresponding to cancers. The the decreased the 1584 cm Raman with increasin corresponding for the amount of the lowered cytochrome c increases with escalating cancer cer aggressiveness. It indicates that cytochrome c plays a important function within the create aggressiveness. It indicates that cytochrome c plays a crucial role inside the development and progression of cancer. We located the dependence from the Raman biomarker ICancers 2021, 13,18 ofand progression of cancer. We identified the dependence of the Raman biomarker I1584 on the lowered cytochrome c vs grade of cancer malignancy which shows that the optimal concentration of cytochrome c which might be needed to keep cellular homeostasis corresponds to the normalized Raman intensity of 0.006 0.003 for the breast tissue and 0.074 0.005 for the brain tissue. The concentrations of your reduced cytochrome c at this level modulate protective, signaling-response pathways, resulting in positive effects on life-history traits. The reduced cytochrome c level above these values triggers a toxic runaway procedure and aggressive cancer improvement. The relation involving the Raman signal intensity at 1584 cm-1 of the reduced cytochrome c vs cancer grade offers a vital cell-physiologic response demonstrating that the lowered cytochrome c operates at low, basal level in normal cells, but it is strongly induced to pretty high levels in pathological cancer states. We discovered that also the Raman intensity on the mixed vibrations of cytochromes c and b (750 and 1126 cm-1 ) improve with aggressiveness. The Raman peaks at 1337 cm-1 corresponding to cytochromes b will not virtually transform with breast and brain cancer aggressiveness. We analyzed the Raman intensity ratio of your peaks at 750 cm-1 and 1126 cm-1 to evaluate the relative contribution of cytochrome c and b in human breast tissue as a function of breast cancer grade malignancy G0-G3 at excitation 532 nm. We located that the relative contribution of cytochrome c is greater than cytochrome b when the grade of malignancy increases.Author Contributions: Conceptualization: H.A.; Funding acquisition: H.A.; Investigation: B.B.P., M.K., J.S., M.B., M.R.; Methodology: H.A., B.B.-P., M.K., J.S.; Writing–original draft: H.A.; Manuscript editing: H.A., B.B.-P., M.K., J.S. All authors have study and agreed for the published version from the manuscript. Funding: This operate was supported by the Nav1.3 web National Science Centre of Poland (Narodowe Centrum Nauki, UMO-2019/33/B/ST4/01961). Institutional Overview Board Statement: The study was conducted as outlined by the suggestions with the Declaration of Helsinki, and approved by the regional Bioethical Committee at the Polish Mother’s Memorial Hospital Study Institute in Lodz (53/216.