Anisms in leukemic B-cells that could alter the phagocytic capacity of macrophages upon CIT. Strategies: The proteomic profile of control and TP53deficient leukemic B-cells, untreated or treated with mafosfamide, was analysed by mass spectrometry. EVs were isolated from control and TP53-deficient leukemic B cells by differential ultracentrifugation and their proteomic content material was evaluated by mass spectrometry. Validation of protein expression was performed by Western Blot and flow cytometry. The measurements of exosomes concentration and size distribution were performed by NanoSight NS300 and ZetaView. Results: 244 of 5785 proteins have been observed to become significantly diverse involving TP53-deficient and control leukemic B-cells, with 159 independent of mafosfamide remedy, 147 associated to mafosfamide and 86 modifications shared involving DMSO and mafosfamide remedy. Enrichment analysis for GO terms showed that TP53-deficient leukemic B-cells exhibited mainly altered expression of proteins associated with EVs. We confirmed that TP53-deficient leukemic Bcells produced greater concentration of EVs and that the EV-protein content differed from handle leukemic B-cells. Notably, 1239 of 2663 proteins were substantially unique amongst TP53-deficient and handle leukemic B-cells, 68 have been exclusively detected in the control-derived EVs and 128 proteins have been only identified inside the TP53-deficient-related EVs Summary/Conclusion: The loss of TP53 drastically modifies the proteomic profile of leukemic B-cells and influences the protein expression of leukemic Bcells upon mafosfamide treatment. In particular, the loss of TP53 regulates the EV-related protein expression and EV production in leukemic B-cellsISEV2019 ABSTRACT BOOKPF02: EVS within the Central and Peripheral Nervous Technique Chairs: Sowmya Yelamanchili; Elena Batrakova Location: Level three, Hall A 15:306:PF02.The impact of exosome purification process around the detection of amyloid in exosomes with Photooxidation-Induced Fluorescence Amplification (PIFA) Youhee Heoa, Min Cheol Parkb, SangYun Kimc, Kwanwoo Shind and Ji Yoon Kange Korea Institute of Sceince and Technology, Seoul, mTORC1 Storage & Stability Republic of Korea; IntekBio, seoul, Republic of Korea; cSeoul national university bundang hospital, seoul, Republic of Korea; dsogang university, soeul, Republic of Korea; eKorea Institute of Science and Technology, Seoul, Republic of Koreab aIntroduction: Blood-based diagnosis of disease making use of exosomes often demands a highly sensitive bioassay to detect rare protein biomarkers. New assay techniques have been suggested to overcome the limitations of a conventional ELISA method including ULK2 medchemexpress digital ELISA or plasmonic ELISA. Nonetheless, these techniques will need a special highly-priced gear using the lengthy approach. We have developed a photo-oxidation-induced fluorescence amplification (PIFA) that could measure significantly less than 1 pg/mL by continuous irradiation on resorufin for the photooxidation of chemi-fluorescent substrate amplex red. This paper demonstrated it can determine Alzheimer’s disease (AD) patient from standard manage (NC) by measuring a low amount of amyloid beta(A) inside the neuronal exosome from plasma samples. Strategies: The level of resorufin was measured by PIFA to evaluate with conventional ELISA. The oligomer A was detected by exact same antibody technique whose capture antibody is exact same as detection antibody to exclude the signals from monomer A. We isolated exosomes from plasma samples (AD:4, NC:4) by 3 approaches: ultracentrifuge(UC), CD9 antibody-coated ma.