And of rBC2LCN. Other hiPSC surface glycan markers wereISEV2019 ABSTRACT BOOKalso detected around the surface of EVs. Ultimately, we developed a Siglec 6/CD327 Proteins MedChemExpress sandwich assay to especially detect hiPSCderived EVs using rBC2LCN and Tim4, which binds to phosphatidylserine (PS). rBC2LCN is valuable for the particular detection of hiPSC-derived EVs. Summary/Conclusion: The EV glycome reflects its cellular origin, which could be a novel target for the improvement of the good quality manage method of stem cells utilised for regenerative medicine. Funding: JST CRESTOF13.Exosomes derived from human MSC mediate monocyte mobilization to orchestrate neovascularization in radiation-induced skin injury Alexandre Ribaulta, Bruno Lhommea, Celine Loinarda, Marc Benderittera, Stephane Flamanta, Ruenn Chai Laib, Sai Kiang Limc and Radia Tamarataamuscle. On top of that, monocyte and macrophage depletion through clodronate therapy totally abrogated the pro angiogenic effect of huMSC-Exo. Summary/Conclusion: This study demonstrates, for the initial time, that huMSC derived exosomes boost the angiogenic procedure within the radiation-induced ischemic tissue by stimulating the mobilization and recruitment of innate cells to the lesion and nurturing neovascularization. These outcomes highlight the idea that huMSC-Exo administration represents a appropriate revolutionary approach for therapeutic angiogenesis in irradiated tissue.OF13.hucMSCs derived exosomes enhance lymphangiogenesis in experimental lymphedema by way of exosomal transfer of Ang-2 and Tie2 Ting Zhao and Yongmin Yan Jiangsu University, Zhenjiang, China (People’s Republic)IRSN, Paris, France; bIMB ASTAR, Singapore, USA; cInstitute of Medical Biology, Agency for Science, Technology and Investigation, Singapore, SingaporeIntroduction: Emerging evidences indicate that extracellular membrane vesicles, which include exosomes, could P-Selectin/CD62P Proteins Formulation recapitulate the therapeutic effects of huMSC. Of note, exosomes displayed marked pro-angiogenic activity, however a greater understanding of their underlying mechanisms of action remained to become defined. This study aims to investigate the mechanisms governing the pro-angiogenic effects of huMSC derived exosomes (huMSC-Exo) within a mouse model of radiation-induced musculo-cutaneous injury. Techniques: Mice reduce limb was exposed to 80Gy X-ray irradiation to induce radiation injury. Following 14 days, mice received an intramuscular injection of 106 human MSCs, 400 MSC-EXO, or PBS. Angiogenesis was estimated by skin perfusion (laser Doppler imaging), immunohistochemistry (CD 31 endothelial marker) and microangiography (barium sulphate). Mice were sacrificed at many time points, and tissues of each irradiated and contralateral limbs have been harvested for histological and biochemical analyses. Bone marrow, spleen and blood had been collected for analysis of inflammatory cells and circulating elements. In vitro assays had been used to validate the pro angiogenic effet of HuMSC- exo. Results: The huMSC-Exo stimulated vascular growth as revealed by the boost in cutaneous blood perfusion, capillary density and angiographic score with stimulation of pro-angiogenic factor levels including VEGF-A and eNOS. In vitro, huMSC-Exo fostered endothelial cells and fibroblast migration within a PI3K/ AKT and TGF-/SMAD2 dependent pathways. Ultimately, huMSC-Exo triggered the mobilization of both Ly6Chi and Ly6Clo monocytes in the spleen along with the bone marrow and their recruitment into the irradiatedIntroduction: Exosomes are smaller biological membrane vesicles secreted by cel.