Anisms in leukemic B-cells that could alter the phagocytic capacity of macrophages upon CIT. Procedures: The proteomic profile of handle and TP53deficient leukemic B-cells, untreated or treated with mafosfamide, was analysed by mass spectrometry. EVs have been isolated from handle and TP53-deficient leukemic B cells by differential ultracentrifugation and their proteomic content was evaluated by mass spectrometry. Validation of protein expression was performed by Western Blot and flow cytometry. The measurements of exosomes concentration and size distribution have been performed by NanoSight NS300 and ZetaView. Outcomes: 244 of 5785 proteins were observed to be drastically various involving TP53-deficient and handle leukemic B-cells, with 159 independent of mafosfamide treatment, 147 related to mafosfamide and 86 modifications shared involving DMSO and mafosfamide remedy. Enrichment evaluation for GO terms showed that TP53-deficient leukemic B-cells exhibited primarily altered expression of proteins linked with EVs. We confirmed that TP53-deficient leukemic Bcells created greater concentration of EVs and that the EV-protein content material differed from manage leukemic B-cells. Notably, 1239 of 2663 proteins have been substantially unique between TP53-deficient and handle leukemic B-cells, 68 have been exclusively detected inside the control-derived EVs and 128 proteins were only found within the TP53-deficient-related EVs Summary/Conclusion: The loss of TP53 drastically modifies the proteomic profile of leukemic B-cells and influences the protein expression of leukemic Bcells upon mafosfamide remedy. Specifically, the loss of TP53 regulates the EV-related protein expression and EV production in leukemic B-cellsISEV2019 ABSTRACT BOOKPF02: EVS within the Central and Peripheral Nervous Method Adiponectin Proteins MedChemExpress Chairs: Sowmya Yelamanchili; Elena Batrakova Place: Level three, Hall A 15:306:PF02.The effect of exosome purification approach on the detection of amyloid in exosomes with Photooxidation-Induced Fluorescence Amplification (PIFA) Youhee Heoa, Min Cheol Parkb, SangYun Kimc, Kwanwoo Shind and Ji Yoon Kange Korea Institute of Sceince and Technologies, Seoul, Republic of Korea; IntekBio, seoul, Republic of Korea; cSeoul national CD147 Proteins Purity & Documentation university bundang hospital, seoul, Republic of Korea; dsogang university, soeul, Republic of Korea; eKorea Institute of Science and Technologies, Seoul, Republic of Koreab aIntroduction: Blood-based diagnosis of illness utilizing exosomes often demands a hugely sensitive bioassay to detect rare protein biomarkers. New assay procedures had been suggested to overcome the limitations of a conventional ELISA system like digital ELISA or plasmonic ELISA. Nevertheless, these techniques have to have a specific highly-priced gear together with the extended process. We have developed a photo-oxidation-induced fluorescence amplification (PIFA) that can measure much less than 1 pg/mL by continuous irradiation on resorufin for the photooxidation of chemi-fluorescent substrate amplex red. This paper demonstrated it might recognize Alzheimer’s disease (AD) patient from normal control (NC) by measuring a low degree of amyloid beta(A) in the neuronal exosome from plasma samples. Methods: The degree of resorufin was measured by PIFA to evaluate with traditional ELISA. The oligomer A was detected by exact same antibody system whose capture antibody is very same as detection antibody to exclude the signals from monomer A. We isolated exosomes from plasma samples (AD:4, NC:four) by 3 techniques: ultracentrifuge(UC), CD9 antibody-coated ma.