Tion, wax, eggs and animal protein materials, presumably within the affresco method. Moreover, wax, eggs and [80]. identified, the presence of which can be as a consequence of prior restoration workanimal glue have been identified, the presence of that is as a consequence of earlier restoration perform [80].Figure 1. The picture of Season in room 42 (the antechamber of the so-called erotic Clindamycin palmitate (hydrochloride) Cancer cubiculum) of Figure 1. The image of Season in space 42 (the antechamber on the so-called erotic cubiculum) of Insula del Centenario, Pompeii, Italy, exactly where sample 42/2 was collected. Insula del Centenario, Pompeii, Italy, exactly where sample 42/2 was collected.A paper has been published on Villa from the Papyri in Herculaneum, to be able to know the presence of an organic binder. The authors discovered egg, by suggests of GC-MS, in some samples, where the pigment required a secco method. Additionally they investigated the presence of wax employed as superficial treatment options in the wall painting. The truth that they didn’t detect beeswax in all the analyzed samples give additional data that the encaustic method was not applied for the realization on the wall paintings in the Vesuvian location [81]. An exciting perform examined the wooden roof and ceiling on the Home of the Telephus Relief in Herculaneum. The purpose of this study was to assess the techniques utilized to decorate the wooden ceiling, employing a multianalytical approach (SEM/EDX, FT-IR spectroscopy, GC/MS and Py-GC/MS). The outcomes showed that a tempera painting has been chosen. Egg was employed as binding medium, as confirmed by the analysis of your proteinaceous fraction and by the presence of cholesterol. In only a single sample, caseinAppl. Sci. 2021, 11,12 ofwas determined, possibly employed as an adhesive. The paint layer was directly applied the wooden ceiling, devoid of a preparatory stratum. A motivating aspect of this paper is the fact that the authors argue that, based on chemical investigations, this can be the first time it has been feasible to prove that egg tempera has been used to decorate wooden architectural elements [82]. Cunhighlights the fact that investigations of ancient Roman wall painting show the difficulty of effectively extracting organic binders. The author considers that sometimes it can be regarded as an affresco painting, because of the lack of outcomes obtained, when the organic binder may very well be present, but not Ethyl pyruvate Biological Activity identified for purely analytical issues. The usage of inappropriate extraction methods for ancient paint supports can cause the transformation of some components of the binder, generating an incomplete characterization of your composition He pointed out that chemical analyses of Roman wall painting have identified other forms of paint binders and that there’s a clear distinction amongst the results of a single research group as well as the other. These aspects may be connected for the difficulty of extracting ancient painting binders and, moreover, the influence of microbiological contamination. The absence of data in most research on the efficiency of extraction actions used in antique samples along with the impact of microbial contamination may give rise to doubts about several of the benefits obtained [8]. Cunet al. (2012) studied eight samples of Roman wall paintings from three archaeological websites in Spain employing complete attenuated reflectance Fourier transform infrared spectroscopy and GC-MS, in an effort to recognize organic binder. It was noticed the samples featured beeswax and soap. These benefits show for the very first time that Roman artists utilized in mural paintings.