At: https://www.frontiersin.org/articles/10.3389/fimmu. 2019.01824/full#supplementary-material
Cell. Mol. Life Sci. (2015) 72:983?97 DOI ten.1007/s00018-014-1730-Cellular and Molecular Life SciencesRESEARCH ARTICLEMitochondrial ferritin, a brand new target for inhibiting neuronal tumor cell proliferationZhen-Hua Shi ?Fang-Fang Shi ?Yue-Qi Wang ?Alex D. Sheftel ?Guangjun Nie ?Ya-Shuo Zhao ?Lin-Hao You ?Yu-Jing Gou ?Xiang-Lin Duan ?Bao-Lu Zhao ?Hong-Meng Xu ?Chun-Yan Li ?Yan-Zhong ChangReceived: 24 July 2014 / Revised: 3 September 2014 / Accepted: five September 2014 / Published on the internet: 12 September 2014 ?The Author(s) 2014. This article is published with open access at Springerlink.comAbstract Mitochondrial ferritin (FtMt) includes a considerable effect around the regulation of cytosolic and mitochondrial iron levels. On the other hand, because of the deficiency of iron regulatory elements (IRE) in FtMt’s gene sequence, the precise function of FtMt remains unclear. Inside the present study, we identified that FtMt substantially inhibited SH-SY5Y cell proliferation and tumor growth in nude mice. Interestingly, excess FtMt did not adversely have an effect on the development of drosophila. Furthermore, we found that the expression of FtMt in human typical brain tissue was drastically higherZ.-H. Shi, F.-F. Shi and Y.-Q. Wang contributed equally to this work. Z.-H. Shi and F.-F. Shi are co-first authors.Electronic supplementary material The on line version of this short article (doi:ten.1007/s00018-014-1730-0) contains supplementary material, which is obtainable to authorized users.Z.-H. Shi ?F.-F. Shi ?Y.-Q. Wang ?Y.-S. Zhao ?L.-H. You ?Y.-J. Gou ?X.-L. Duan ?B.-L. Zhao ?Y.-Z. Chang Important Fmoc-Gly-Gly-OH Epigenetic Reader Domain Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Regular University, Shijiazhuang 050024, Hebei, China Z.-H. Shi e-mail: [email protected] Z.-H. Shi ?F.-F. Shi ?Y.-Q. Wang ?Y.-S. Zhao ?L.-H. You ?Y.-J. Gou ?X.-L. Duan ?B.-L. Zhao ?Y.-Z. Chang Important Laboratory of Molecular and Cellular Biology of Ministry of Education, College of Life Science, Hebei Typical University, Shijiazhuang 050024, Hebei, China Z.-H. Shi ?F.-F. Shi ?Y.-Q. Wang ?Y.-S. Zhao ?L.-H. You ?Y.-J. Gou ?X.-L. Duan ?B.-L. Zhao ?Y.-Z. Chang ( ) Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Standard University, Shijiazhuang 050024, Hebei, China e-mail: [email protected] that of neuroblastoma, but not larger than that of neurospongioma. CM10 Metabolic Enzyme/Protease Nonetheless, the expression of transferrin receptor 1 is entirely opposite. We for that reason hypothesized that increased expression of FtMt may well negatively impact the vitality of neuronal tumor cells. For that reason, we further investigated the underlying mechanisms of FtMt’s inhibitory effects on neuronal tumor cell proliferation. As expected, FtMt overexpression disturbed the iron homeostasis of tumor cells and substantially downregulated the expression of proliferating cell nuclear antigen. Additionally, FtMt impacted cell cycle, causing G1/S arrest by modifying the expression of cyclinD1, cyclinE, Cdk2, Cdk4 and p21. Remarkably, FtMt strongly upregulated the expression from the tumor suppressors, p53 and N-myc downstream-regulated gene-1 (NDRG1), but dramatically decreased C-myc, N-myc and p-Rb levels. This study demonstrates for the first time a new role and mechanism for FtMt in theA. D. Sheftel University of Ottawa Heart Institute, 40 Ruskin St., Ottawa, ON K1Y 4W7, Canada G. Nie CAS Essential Laboratory for Biomedical Effects of Nanomaterial.