Andong Yang (University of Virginia, USA) plus the RIKEN BRC (Ibaraki, J) for the viral and Chlortetracycline site murine IL-10 cDNA clones, respectively. We are grateful to Dr. Johannes Stadlmann (University of Vienna) for the glycan evaluation. We thank Dr. Richard Twyman for crucial reading on the manuscript. This operate was funded by the EU FP6/Pharma-Planta (LSHB-CT-2003-503565) and was supported by the price action `Molecular Farming: Plants as a Production Platform for Higher Value Proteins’ FA0804. Author facts Division of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy. 2Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Forckenbeckstrasse 6, 52074, Aachen, Germany. 3Present address: Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany.Authors’ contributions LB participated inside the design from the study, performed the induction experiments and IL-10 and DsRed measurements, analyzed the data and wrote the manuscript. TR conceived the cloning technique and generated the new vectors, performed the development curves evaluation and helped drafting the manuscript. AS selected and characterized the repressor line and critically revised the manuscript. FS generated all transgenic lines and helped interpreting the results. MP and SS conceived the study, participated in its design and coordination, interpretation of information and vital reading of your manuscript. All authors read and authorized the final manuscript. Received: 3 February 2012 Accepted: 11 July 2012 Published: 11 July 2012 References 1. Schiermeyer A, Schillberg S: Pharmaceuticals. In Genetic modification of plants: agriculture, horticulture and forestry. Edited by Kempken F, Jung C. Berlin Heidelberg: Springer; 2010:221?35. 2. Faye L, Gomord V: Good results stories in molecular farming-a brief overview. Plant Biotechnol J 2010, eight(five):525?28. three. Bock R, Warzecha H: Solar-powered factories for new vaccines and antibiotics. Trends Biotechnol 2010, 28(five):246?52. four. Peters J, Stoger E: Transgenic crops for the production of recombinant vaccines and anti-microbial antibodies. Hum Vaccin 2011, 7:three. five. Tremblay R, Wang D, Jevnikar AM, Ma S: Tobacco, a very efficient green p-Tolualdehyde manufacturer bioreactor for production of therapeutic proteins. Biotechnol Adv 2010, 28(two):214?21. 6. Obembe OO, Popoola JO, Leelavathi S, Reddy SV: Advances in plant molecular farming. Biotechnol Adv 2011, 29(two):210?22. 7. Zhong J: Plant cells (Advances in biochemical engineering-biotechnology), Volume 72. Berlin Heidelberg: Springer; 2001. eight. Schiermeyer A, Schinkel H, Apel S, Fischer R, Schillberg S: Production of Desmodus rotundus salivary plasminogen activator alpha1 (DSPAalpha1) in tobacco is hampered by proteolysis. Biotechnol Bioeng 2005, 89(7):848?58. 9. Huang TK, Plesha MA, McDonald KA: Semicontinuous bioreactor production of a recombinant human therapeutic protein applying a chemically inducible viral amplicon expression program in transgenic plant cell suspension cultures. Biotechnol Bioeng 2010, 106(3):408?21. ten. Sorrentino A, Schillberg S, Fischer R, Porta R, Mariniello L: Molecular farming of human tissue transglutaminase in tobacco plants. Amino Acids 2009, 36(4):765?72.11. Avesani L, Vitale A, Pedrazzini E, Devirgilio M, Pompa A, Barbante A, Gecchele E, Dominici P, Morandini F, Brozzetti A, Falorni A, Pezzotti M: Recombinant human GAD65 accumulates to high levels in transgenic tobacco plants when expressed as an enzymatically inactive mutant. Plant Biotechno.