The mouse Pdlim5 gene-trapped ES mobile line (XH199) was obtained from the Mutant Mouse Regional Useful resource Centre. The Pdlim5 gene, which is made up of 13 exons, specifies a PDZ domain and 3 LIM domains
Exon two of Pdlim5 specifies the finish of the PDZ domain, whilst exon nine specifies the starting of the initially LIM domain. The gene-lure vector, pGT1lxf that contains the Engrailed…