Cells were being pre-incubated for ten min with coelenterazine and then stimulated with escalating ligand concentrations
Immunoprecipitation of IR and IR/IGF1R hybrids was executed working with a monoclonal anti-IR antibody (CT1) covalently certain to sepharose beads . Immunoblotting was done utilizing anti-IR (Santa Cruz C-19), anti-IGF1R…