The NLS-I-SceI molecule is made up of 36SV40 NLS, an HA tag epitope and the indigenous I-SceI molecule as proven in Fig. 1 B. 36SV40 NLS is extremely potent for nuclear localization, and HA tag epitope can be used to detect NLS-I-SceI molecule distribution in cells as soon as it was expressed in cytoplasm
The NLS-I-SceI CDS was optimized for equally porcine and murine codon use tastes (the NLS-I-SceI CDS and amino acid sequence have been revealed in Fig. S1 and S2). Significant quality…