The residue indicated with `X’ (in bold) in the sequence was complementary to the incoming nucleotide. Different oligonucleotides with just about every of the four nucleotides at this position were being employed

Nucleotides, oligonucleotides and industrial enzymes dADP, dCDP, dGDP, dTDP (dNDPs) and Damp were acquired from MP Biomedicals. AZTTP was obtained from Moravek Biochemicals. In experiments comparing polymerase action with Moist,…