Motif details within a sequence-wide manner. GO terms corresponding to these Interpro-domains, have been then transferred for the sequences and merged with currently existent GO terms. GO terms have been modulated employing the annotation augmentation tool ANNEX [34], followed by GOSlim. As described in the Blast2GO tutorial ”the ANNEX method makes use of uni-vocal relationships in between GO terms from unique GO categories to add implicit annotation and GOSlim is a lowered version of the GO that contains a selectedPLOS A single | www.plosone.orgOlive Fruit Fly Transcriptome-Detoxification Genesperformed to evaluate the branch strength of each and every tree. The resulting tree was midpoint rooted and edited with MEGA 5.Capmatinib 0 computer software [39].Table S6 Sequence name, nucleotide and amino acid length and sequence, class and class identification method of contigs encoding for putative B. oleae GSTs. (XLSX) Table S7 Sequence name, nucleotide and amino acid length and sequence, clade and clade identification technique of contigs encoding for putative B. oleae CCEs. (XLSX) Table S8 Sequence name, nucleotide and amino acid length and sequence, subfamily and subfamily identification approach of contigs encoding for putative B. oleae ABC transporters. (XLSX) File S1 dN/dS (v) evaluation in B. oleae/B. dorsalis ortholog pairs (P450s, GSTs, CCEs).Daclatasvir (DOCX) File S2 A number of alignment of B.PMID:27017949 oleae P450, GST, CCESupporting InformationFigure SBlast statistics. (A) E-value and (B) percentagesimilarity. (TIF)Figure S2 Phylogenetic evaluation of B. oleae putative ABC transporters (subfamily B). Four B. oleae ABC sequences are clustered in B subfamily. Agam: Anopheles gambiae, Amel: Apis mellifera, Bmor: Bombyx mori, [42] Dpul: Daphnia pulex [43], Dmel: Drosophila melanogaster, Hsap: Homo sapiens, Tcas: Tribolium castaneum. (TIF)Phylogenetic analysis of B. oleae putative ABC transporters (subfamily D). A single B. oleae ABC sequence is clustered in D subfamily. Agam: Anopheles gambiae, Amel: Apis mellifera, Dmel: Drosophila melanogaster, Dpul: Daphnia pulex, Hsap: Homo sapiens. (TIF)Figure S3 Figure S4 Phylogenetic evaluation of B. oleae putative ABC transporters (subfamilies E and F). One particular B. oleae ABC sequence is clustered in E and 1 in F subfamily. Agam: Anopheles gambiae, Amel: Apis mellifera, Bmor: Bombyx mori, Dmel: Drosophila melanogaster, Dpul: Daphnia pulex, Hsap: Homo sapiens, Tcas: Tribolium castaneum. (TIF) Table S1 Top rated BLAST hits within the NCBI nr database. Only contigs having a blast hit are presented. (XLSX) Table S2 Gene ontology categorization benefits. Onlycontigs which returned a single or a lot more GO terms are presented. (XLSX)Table S3 Distribution of putative enzymes in KEGGpathways. (XLSX)Table S4 Sequence name, nucleotide and amino acidand ABC transporters (B, D, E and F subfamilies) protein sequences using a representative dataset of their counterparts in other species. Aaeg: Aedes aegypti, Acal: Anisopteromalus calandrae, Aech: Acromyrmex echinatior, Agam: Anopheles gambiae, Agos: Aphis gossypii, Amel: Apis mellifera, Apol: Antheraea polyphemus, Bdor: Bactrocera dorsalis (“JF” sequences obtained from [12]), Bger: Blatella germanica, Bmor: Bombyx mori, Bole: Bactrocera oleae, Bpap: Bactrocera papaya Btab: Bemisia tabaci, Cfum: Choristoneura fumiferana, Cpip: Culex pipiens, Ctar: Culex tarsalis, Ctri: Culex tritaeniorhynchus, Dmel: Drosophila melanogaster, Dpas:Depressaria pastinacella, Dpul: Daphnia pulex, Dpun: Diploptera punctata, Harm: Helicoverpa armigera, Hirr: Haematobia irritans, Hsap: Homo sapiens,.