Ng process. In addition, EDX alysis indicated the presence of several inorganic elemental components in released PM (zinc, copper, titanium, cerium, silicon, calcium, and sulfur). The pie chart in Supplementary Figure SD shows the ICPMS alysis on PEPs and it confirms the presence of copper, cerium, chromium, nickel, iron, and titanium. These observations confirm the fact that the ENMs incorporated in printer toner formulations had been aerosolized during the printing procedure. Interested readers can find the comprehensive PCM characterization particulars on toner powder and PEPs in current publications from Pirela et al. (b). Step PEPs SB-366791 site extraction From Collecting Media The collected PEPs (each PM. and PM. fractions) were extracted from HCCI impactor substrates. The aqueous extraction protocol described previously inside the `Methods’ section was utilized. As shown in Supplementary Table S, DSE under Jml was used for PEPs aqueous extraction for each PM. and PM. fractions. Control experiments (with out LCPM) indicated that delivered extraction PubMed ID:http://jpet.aspetjournals.org/content/121/4/414 energy beneath Jml had no influence on mass variations of blank Teflon and PUF substrates, an indication that both forms of substrates had been intact in the course of the extraction approach and no fibersdebris were released. Making use of the protocol described in Figure, the aqueous extraction was effective to extract collected LCPM mass from collection media. Step PEPs Dispersion Preparation and Characterization Vital DSE, DSEcr, was located to be Jml (Table ). Observed values of f were strongly damaging for the PM. PEPs size fraction in DI water (. mV) and became optimistic when dispersed in SABM (. mV). The opposite was observed for the larger PEPs counterpart (PM.), whose f was mV in DI water and remained damaging when suspended in SABM (. mV). The colloidal stability of your particle suspensions was subsequently evaluated h postsonication to DSEcr. The size of PEPs (PM.) suspended in SABM remained steady with an 
typical diameterRESULTSResults from the application on the SEDD methodology for the case research are presented as follows.Case Study : Release of ENMs In the course of Use of a noEbled Toner Utilised in Laser Printers: EHS Implications of PEPsStep Aerosolized Monitoring, Sampling, and PCM Characterization of PEPs Supplementary Figure SA shows a unimodal particle size distribution throughout the printing procedure. Mean particleTOXICOLOGICAL SCIENCES,, Vol., No.TABLE. Characterization (d(h,zave), PdI and zeta) of extracted particles in DI water at DSEcr and just after particle dispersion in SABM Material label In DI water d(h, zave) (nm) PdI PEPs PM. PEPs PM. TNEPs PM. TNEPs PM…… Zeta f (mV)…. In SABM d(h, zave) (nm) PdI.. Zeta f (mV) Dispersion stability (h post dispersion) d(h, zave) (nm). PdI…. Zeta f (mV)………..Stability of particles in SABM was determined following h, respectively. PEPs data employed with permission from Pirela et al. (a, ). DSEcr for PEPs was Jml and for TNEPs was Jml.TABLE. 
Mass, surface region and particle concentration as cellular dose metric, depending on administered and deposited doses for all particles dispersed in SABM Material Successful density, qe (gcm).. Deposition fraction continual, a (h).. t (h) Administered dose at t h Mass (lgml)…. SA (cm ).E.E.E.E Delivered dose at t h Mass (lg).. SA (cm).E.E.E.E Particles cm.E.E.E.E PEPs PM. PEPs PM. TNEPs PM. TNEPS PMThe dose estimates derived from the VCMISDD model calculations. SA, Surface area. Note Surface location and particle MC-LR number concentration dose metrics are according to VCMISDD mode.Ng method. Furthermore, EDX alysis indicated the presence of several inorganic elemental components in released PM (zinc, copper, titanium, cerium, silicon, calcium, and sulfur). The pie chart in Supplementary Figure SD shows the ICPMS alysis on PEPs and it confirms the presence of copper, cerium, chromium, nickel, iron, and titanium. These observations confirm the truth that the ENMs incorporated in printer toner formulations have been aerosolized during the printing procedure. Interested readers can find the substantial PCM characterization information on toner powder and PEPs in current publications from Pirela et al. (b). Step PEPs Extraction From Collecting Media The collected PEPs (each PM. and PM. fractions) have been extracted from HCCI impactor substrates. The aqueous extraction protocol described previously within the `Methods’ section was utilised. As shown in Supplementary Table S, DSE under Jml was made use of for PEPs aqueous extraction for each PM. and PM. fractions. Manage experiments (devoid of LCPM) indicated that delivered extraction PubMed ID:http://jpet.aspetjournals.org/content/121/4/414 power under Jml had no influence on mass variations of blank Teflon and PUF substrates, an indication that each types of substrates were intact for the duration of the extraction method and no fibersdebris have been released. Working with the protocol described in Figure, the aqueous extraction was effective to extract collected LCPM mass from collection media. Step PEPs Dispersion Preparation and Characterization Important DSE, DSEcr, was found to become Jml (Table ). Observed values of f have been strongly unfavorable for the PM. PEPs size fraction in DI water (. mV) and became good when dispersed in SABM (. mV). The opposite was observed for the bigger PEPs counterpart (PM.), whose f was mV in DI water and remained negative when suspended in SABM (. mV). The colloidal stability in the particle suspensions was subsequently evaluated h postsonication to DSEcr. The size of PEPs (PM.) suspended in SABM remained steady with an average diameterRESULTSResults in the application in the SEDD methodology for the case research are presented as follows.Case Study : Release of ENMs Through Use of a noEbled Toner Applied in Laser Printers: EHS Implications of PEPsStep Aerosolized Monitoring, Sampling, and PCM Characterization of PEPs Supplementary Figure SA shows a unimodal particle size distribution all through the printing method. Imply particleTOXICOLOGICAL SCIENCES,, Vol., No.TABLE. Characterization (d(h,zave), PdI and zeta) of extracted particles in DI water at DSEcr and just after particle dispersion in SABM Material label In DI water d(h, zave) (nm) PdI PEPs PM. PEPs PM. TNEPs PM. TNEPs PM…… Zeta f (mV)…. In SABM d(h, zave) (nm) PdI.. Zeta f (mV) Dispersion stability (h post dispersion) d(h, zave) (nm). PdI…. Zeta f (mV)………..Stability of particles in SABM was determined following h, respectively. PEPs information used with permission from Pirela et al. (a, ). DSEcr for PEPs was Jml and for TNEPs was Jml.TABLE. Mass, surface area and particle concentration as cellular dose metric, based on administered and deposited doses for all particles dispersed in SABM Material Successful density, qe (gcm).. Deposition fraction continual, a (h).. t (h) Administered dose at t h Mass (lgml)…. SA (cm ).E.E.E.E Delivered dose at t h Mass (lg).. SA (cm).E.E.E.E Particles cm.E.E.E.E PEPs PM. PEPs PM. TNEPs PM. TNEPS PMThe dose estimates derived in the VCMISDD model calculations. SA, Surface area. Note Surface area and particle number concentration dose metrics are determined by VCMISDD mode.