mouse hippocampi ended up dissected in HBSS dissecting media that contains four mM NaHCO3 (7.5%) and 10 mM HEPES buffer
Hippocampal neuron cultures were prepared from P1 CD-1 mice or transgenic mice more than expressing human a-syn tagged to GFP at its C-terminus (h-a-syn-GFP tg) . Briefly, Neurons ended up…