For trypsin treatment method prior to the import reaction, mitochondria ended up incubated with trypsin (25 g/ml) for 15 min on ice, adopted by more incubation for ten min right after addition of twenty excessive of soy trypsin inhibitor

Movement cytometry was done using a FACSCalibur (Becton Dickinson). Apoptosis was inhibited with Q-VD-OPh (QVD, 10M, SM Biochemical) added 30min prior or alongside one another with stimulation with 4HT. Stability…